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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/12/2013 |
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Data da última atualização: |
10/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C. |
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Afiliação: |
ESALQ/USP; ESALQ/USP; ESALQ/USP; MARCELO FIDELES BRAGA, CPAC; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP. |
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Título: |
Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/aab.12028 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping. MenosOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of w... Mostrar Tudo |
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Thesagro: |
Maracujá; Marcador genético; Marcador molecular; Melhoramento genético vegetal; Polimorfismo Genético. |
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Thesaurus Nal: |
Genetic improvement; Genetic markers; Genetic polymorphism; Passiflora alata; Passion fruits. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02903naa a2200373 a 4500
001 1973873
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008 2013 bl uuuu u00u1 u #d
022 $a0003-4746
024 7 $a10.1111/aab.12028$2DOI
100 1 $aPEREIRA, G. S.
245 $aMolecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species.
260 $c2013
520 $aOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping.
650 $aGenetic improvement
650 $aGenetic markers
650 $aGenetic polymorphism
650 $aPassiflora alata
650 $aPassion fruits
650 $aMaracujá
650 $aMarcador genético
650 $aMarcador molecular
650 $aMelhoramento genético vegetal
650 $aPolimorfismo Genético
700 1 $aNUNES, E. S.
700 1 $aLAPERUTA, L. D. C.
700 1 $aBRAGA, M. F.
700 1 $aPENHA, H. A.
700 1 $aDINIZ, A. L.
700 1 $aMUNHOZ, C. F.
700 1 $aGAZAFFI, R.
700 1 $aGARCIA, A. A. F.
700 1 $aVIEIRA, M. L. C.
773 $tAnnals of Applied Biology$gv. 162, n. 3, p. 347-361, 2013.
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| Registros recuperados : 4 | |
| 3. |  | AMORIM, M. B.; SCHLINDWEIN, J. A.; PEREIRA, E. C. F.; KESTER, A. N.; MARCOLAN, A. L. Doses, modos de aplicação de calcário e índices de acidez do solo para produção de soja e milho na Região da Zona da mata de Rondônia: respostas das plantas às doses e modos de aplicação de calcário em solo cultivado no sistema plantio direto. Revista Pesquisa e Criação, Porto Velho, n. 10, Suplemento especial, p. 509-516, 2011. Edição dos anais do 20. Seminário Final de Iniciação Científica.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| 4. | | BENTO, G. F.; SCHROEDER, N.; SCHLINDWEIN, J. A.; PEREIRA, E. C. F.; MARCOLAN, A. L. Densidade aparente do dolo submetido a diferentes sistemas de preparo do solo e plantio, e diferentes sistemas de rotação de culturas. In: SIMPÓSIO DE CIÊNCIA DO SOLO DA AMAZÔNIA OCIDENTAL, 1.; ENCONTRO DE LABORATÓRIOS DA AMAZÔNIA OCIDENTAL, 2012, Humaitá, AM. [Anais...]. Humaitá, AM: SBCS, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 4 | |
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